| Field | Specification |
|---|---|
| Target | |
| Alternative names | Melanotransferrin; Melanoma-associated antigen p97; CD antigen CD228; MELTF; MAP97; MFI2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MFI2/MELTF recombinant protein (Position: A60-D647). Human MFI2/MELTF shares 86.7% amino acid (aa) sequence identity with mouse MFI2/MELTF. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects melanotransferrin (gene MELTF) in human, mouse and rat samples and is validated for Western blot, IHC and ELISA.
Melanotransferrin (MELTF, also called p97 or CD228) is a transferrin-family protein attached to the cell surface by a GPI anchor. It binds a single iron atom and has been implicated in cellular iron uptake. It was first identified as a melanoma-associated antigen and is studied in iron biology and tumor cell biology.
| Target | Melanotransferrin (gene MELTF; UniProt P08582, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human MFI2/MELTF fragment (Ala60–Asp647), expressed in E. coli. Sequence identity with the mouse ortholog: 86.7%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Found predominantly in human melanomas and in certain fetal tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
