| Field | Specification |
|---|---|
| Target | |
| Alternative names | Methionine aminopeptidase 1D, mitochondrial; MAP 1D; MetAP 1D; Methionyl aminopeptidase type 1D, mitochondrial; Peptidase M 1D; METAP1D; MAP1D |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human METAP1D recombinant protein (Position: Q36-A335). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects methionine aminopeptidase 1D, mitochondrial (gene METAP1D) in human, mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 37.1 kDa, and the supplier reports an observed band at about 37 kDa.
METAP1D is a mitochondrial methionine aminopeptidase that removes the initiator methionine from newly made proteins when the next residue is small. This processing influences the stability and maturation of mitochondrial proteins. METAP1D is studied in mitochondrial protein processing and has been examined in colon cancer.
| Target | Methionine aminopeptidase 1D, mitochondrial (gene METAP1D; UniProt Q6UB28, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 37.1 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells, human K562 cells, human 293T cells, human HepG2 cells, human THP-1 cells, human HeLa cells, rat liver tissue, rat RH35 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human METAP1D fragment (Gln36–Ala335), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places methionine aminopeptidase 1D, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
