| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ribosome assembly protein METTL17, mitochondrial; False p73 target gene protein; Methyltransferase 11 domain-containing protein 1; Methyltransferase-like protein 17; Protein RSM22 homolog, mitochondrial; METTL17; METT11D1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human METTL17 recombinant protein (Position: K129-R429). Human METTL17 shares 86.8% amino acid (aa) sequence identity with mouse METTL17. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ribosome assembly protein METTL17, mitochondrial in human samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 50.7 kDa, and the supplier reports an observed band at about 64 kDa.
METTL17 is a mitochondrial assembly factor for the small subunit of the mitochondrial ribosome. It binds between the head and body of the small subunit during its assembly, and although it keeps a methyltransferase-like fold that binds S-adenosylmethionine, it appears to have lost catalytic activity. METTL17 is studied in mitochondrial translation.
| Target | Ribosome assembly protein METTL17, mitochondrial (gene METTL17; UniProt Q9H7H0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 50.7 kDa |
| Observed MW | 64 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U2OS cells, human HepG2 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Recombinant human METTL17 fragment (Lys129–Arg429), expressed in E. coli. Sequence identity with the mouse ortholog: 86.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ribosome assembly protein METTL17, mitochondrial in the mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
