| Field | Specification |
|---|---|
| Target | |
| Alternative names | N; 6; -adenosine-methyltransferase catalytic subunit METTL3; Methyltransferase-like protein 3; hMETTL3; N(6)-adenosine-methyltransferase 70 kDa subunit; MT-A70; METTL3; MTA70 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human METTL3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21M06 is a rabbit monoclonal antibody that recognizes N(6)-adenosine-methyltransferase catalytic subunit METTL3 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 64.5 kDa, and the supplier reports an observed band at about 70 kDa.
METTL3 is the catalytic subunit of the main m6A methyltransferase complex, which it forms with METTL14 and WTAP. It installs N6-methyladenosine, the most abundant internal modification of mRNA, thereby affecting mRNA splicing, export, stability and translation. METTL3 is widely studied in development, stem cell differentiation, immunity and cancer.
| Target | N(6)-adenosine-methyltransferase catalytic subunit METTL3 (gene METTL3; UniProt Q86U44, human) |
|---|---|
| Clone | 21M06 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 64.5 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human PC-3 cells, human HepG2 cells, human A549 cells, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human METTL3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places N(6)-adenosine-methyltransferase catalytic subunit METTL3 in the nucleus, nucleus speckle and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed at low level. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
