| Field | Specification |
|---|---|
| Target | |
| Alternative names | Major facilitator superfamily domain-containing protein 12; MFSD12; C19orf28 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MFSD12 recombinant protein (Position: Q66-H443). Human MFSD12 shares 86.8% amino acid (aa) sequence identity with mouse MFSD12. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects major facilitator superfamily domain-containing protein 12 (gene MFSD12) in human samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 52.1 kDa, and the supplier reports an observed band at about 52 kDa.
MFSD12 is a major facilitator superfamily transporter that imports cysteine into melanosomes and lysosomes. In melanocytes, this cysteine supply supports production of red-yellow pheomelanin, so MFSD12 influences skin and hair pigmentation. In other cells it helps maintain lysosomal cystine and cysteine levels, and it is studied in pigmentation and lysosome biology.
| Target | Major facilitator superfamily domain-containing protein 12 (gene MFSD12; UniProt Q6NUT3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.1 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human SiHa cells, human A549 cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human testicular seminoma tissue, human colon adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human MFSD12 fragment (Gln66–His443), expressed in E. coli. Sequence identity with the mouse ortholog: 86.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places major facilitator superfamily domain-containing protein 12 in the melanosome membrane and lysosome membrane. Tissue expression noted by UniProt (human): Widely expressed, with high expression in primary melanocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kai Sun et al. (2025) MFSD12 promotes proliferation, metastasis and invasion of hepatocellular carcinoma cells and its potential correlation with HAVCR2/LGALS9 immune checkpoint axis. Frontiers in Immunology. 10.3389/fimmu.2025.1681887
