Anti-MFSD3 Antibody Picoband®

SKU:BHA21005865
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-MFSD3 antibody (Rabbit polyclonal, IgG). Validated for Western blot, flow cytometry and ELISA. Reactivity: Human. Recognizes solute carrier family 33 member 2. Observed band in Western blot: about 43 kDa.
Target MFSD3
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB, IHC, Flow, ELISA
Options selector
Catalog no. Size Conjugation
A19348-2 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 100 ug/vial; Conjugation (12) - Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647, Carrier Free
    • 100 ug/vial / Unconjugated; 100 ug/vial / Carrier Free: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
      Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
      Form: Lyophilized
      Applications: WB,Flow Cytometry,ELISA
      Application details: Western blot, 0.25-0.5 μg/ml, Human
      Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
      ELISA, 0.1-0.5 μg/ml, -
    • 100 ug/vial / Biotin; 100 ug/vial / Cy3; 100 ug/vial / Fluoro488; 100 ug/vial / Fluoro550; 100 ug/vial / Fluoro594; 100 ug/vial / FITC; 100 ug/vial / APC; 100 ug/vial / PE; 100 ug/vial / Fluoro647: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Form: Liquid
      Applications: WB,IHC,ELISAFlow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cells
    • 100 ug/vial / HRP: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Form: Liquid
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
  • Lead time: varies by selected option; please contact us for current fulfillment timing.
  • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.; At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MFSD3
Alternative names 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
UniProt # Q96ES6
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Flow Cytometry
  • ELISA
Immunogen E.coli-derived human MFSD3 recombinant protein (Position: M1-A381). Human MFSD3 shares 79.5% and 78.4% amino acid (aa) sequence identity with mouse and rat MFSD3, respectively.
Molecular weight 42.7 kDa
Purification Immunogen affinity purified.
Reconstitution Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Cellular localization Cytoplasm. Nucleus.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Catalog no. (Mfr.) A19348-2
Main SKU BHA21005865
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody targets solute carrier family 33 member 2 (gene SLC33A2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 42.7 kDa, and the supplier reports an observed band at about 43 kDa.

SLC33A2 is a member of solute carrier family 33, whose best-characterized member, SLC33A1, transports acetyl-CoA into the endoplasmic reticulum. The function of SLC33A2 itself has not been fully characterized.

Target Solute carrier family 33 member 2 (gene SLC33A2; UniProt Q96ES6, human)
Host / Clonality / Isotype Rabbit / Polyclonal / IgG
Reactivity Human
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4
Calculated MW 42.7 kDa
Observed MW 43 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.25–0.5 µg/mL (Human)
Flow cytometry (fixed cells) 1–3 µg/1×106 cells (Human)
ELISA 0.1–0.5 µg/mL

Samples with a confirmed band (WB): human A549 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.

Samples with a confirmed signal (flow cytometry): 293T cells.

Immunogen

Recombinant human MFSD3 fragment (Met1–Ala381), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 79.5% and 78.4%, respectively.

Reactivity Notes

The supplier lists reactivity with human. UniProt places solute carrier family 33 member 2 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

For research use only; not for diagnostic or therapeutic procedures. Handle with standard laboratory precautions.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.25–0.5 µg/mL (Human); Flow cytometry (fixed cells) 1–3 µg/1×10⁶ cells (Human); ELISA 0.1–0.5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 42.7 kDa; observed band at about 43 kDa.
Q.What immunogen was used to raise this antibody?
A.Recombinant human MFSD3 fragment (Met1–Ala381), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 79.5% and 78.4%, respectively.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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