| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I; MGST1; GST12; MGST |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MGST1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27M16 is a rabbit monoclonal antibody that recognizes microsomal glutathione S-transferase 1 (gene MGST1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 17.6 kDa, and the supplier reports an observed band at about 18 kDa.
Microsomal glutathione S-transferase 1 (MGST1) is a membrane-bound enzyme found mainly in the endoplasmic reticulum and outer mitochondrial membrane. It joins reduced glutathione to a wide range of hydrophobic electrophiles and also has peroxidase activity toward lipid hydroperoxides, so it is studied in detoxification and protection against oxidative stress.
| Target | Microsomal glutathione S-transferase 1 (gene MGST1; UniProt P10620, human) |
|---|---|
| Clone | 27M16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 17.6 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human RT4 cells, human A549 cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MGST1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places microsomal glutathione S-transferase 1 in the endoplasmic reticulum membrane and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Highly expressed in liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
