Anti-MHC class I HLA-A Rabbit Monoclonal Antibody

SKU:BHA21008544
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-HLA-A antibody (Rabbit monoclonal, clone AOID-8, Rabbit IgG). Validated for Western blot, immunohistochemistry, immunofluorescence and flow cytometry. Reactivity: Human. Observed band in Western blot: about 41 kDa.
Target HLA-A
clone number AOID-8
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB, IHC, ICC, IF, Flow
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Options selector
Catalog no. Size Conjugation
M00194-2 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC,ICC,IF,Flow Cytometry
      Application details: WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HLA-A
Alternative names HLA class I histocompatibility antigen, A-1 alpha chain;MHC class I antigen A*1;HLA-A;HLAA;
Gene ID 3106
UniProt # P30443/P01889/P01891/P01892/P13746/P16188/P30450
Host Rabbit
Clone AOID-8
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
Immunogen A synthesized peptide derived from human MHC class I
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Membrane; Single-pass type I membrane protein.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00194-2
Main SKU BHA21008544
Rabbit Monoclonal

Product Overview

Clone AOID-8 is a rabbit monoclonal antibody that recognizes HLA-A in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry.

Target HLA-A (UniProt P30443/P01889/P01891/P01892/P13746/P16188/P30450)
Clone AOID-8
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Observed MW 41 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200
Immunocytochemistry / Immunofluorescence 1:50–1:200
Flow cytometry 1:50

Immunogen

Synthetic peptide based on the sequence of human MHC class I.

Reactivity Notes

The supplier lists reactivity with human.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: observed band at about 41 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human MHC class I.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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