Anti-MHC class I HLA-A Rabbit Monoclonal Antibody

Overview

This product is an anti-HLA-A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AOID-8; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-MHC class I HLA-A Rabbit Monoclonal Antibody catalog # M00194-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: HLA-A (HLA class I histocompatibility antigen, A-1 alpha chain).
• Antibody format: Monoclonal; clone AOID-8; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

HLA-A (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Involved in the presentation of foreign antigens to the immune system. Reported cellular localization context: Membrane; Single-pass type I membrane protein. Tissue expression notes (as provided): Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. .

Research relevance and current trends

• Research context keywords from the source record include: Adaptive Immunity,B Cells,Cancer,Cancer Metabolism,Hematopoietic Progenitors,Immunology,Lymphoid,Metabolism,Metabolism Processes,Myeloid,Pathways and Processes,Response To Hypoxia,Stem Cells,Surface Molecules,T Lymphocytic Lineage.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate HLA-A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HLA-A expression by Western blot in cell or tissue lysates, Detect HLA-A in FFPE tissue sections by immunohistochemistry, Localize HLA-A by immunofluorescence/immunocytochemistry in cultured cells, Quantify HLA-A-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 45 kDa; calculated MW: 40846 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 45 kDa
• Cellular localization (provided): Membrane; Single-pass type I membrane protein.
• Tissue details (provided): Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.