| Field | Specification |
|---|---|
| Target | |
| Alternative names | MHC class I polypeptide-related sequence A; MIC-A; MICA; PERB11.1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MICA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25M78 is a rabbit monoclonal antibody that recognizes MHC class I polypeptide-related sequence A (gene MICA) in human samples and is validated for Western blot. The predicted molecular weight is 42.9 kDa, and the supplier reports an observed band at about 42–70 kDa.
MICA is a stress-induced, MHC class I-like membrane protein that does not present antigen. Infection, DNA damage and transformation increase its surface expression, where it binds the activating receptor NKG2D on natural killer cells, γδ T cells and CD8+ T cells, so it is widely studied in tumor immune surveillance and innate immunity.
| Target | MHC class I polypeptide-related sequence A (gene MICA; UniProt Q29983, human) |
|---|---|
| Clone | 25M78 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.9 kDa |
| Observed MW | 42–70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MICA.
Reactivity Notes
The supplier lists reactivity with human. UniProt places MHC class I polypeptide-related sequence A in the cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed with the exception of the central nervous system where it is absent. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
