| Field | Specification |
|---|---|
| Target | |
| Alternative names | [F-actin]-monooxygenase MICAL3; Molecule interacting with CasL protein 3; MICAL-3; MICAL3; KIAA0819; KIAA1364 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MICAL3 recombinant protein (Position: K458-L1592). Human MICAL3 shares |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes [F-actin]-monooxygenase MICAL3 in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
MICAL3 is a flavin monooxygenase that oxidizes specific methionine residues on actin, causing actin filaments to disassemble. It also acts as a Rab effector in vesicle trafficking, supporting the docking and fusion of exocytic vesicles, and it is required for cytokinesis.
| Target | [F-actin]-monooxygenase MICAL3 (gene MICAL3; UniProt Q7RTP6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human MICAL3 fragment (Lys458–Leu1592), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places [F-actin]-monooxygenase MICAL3 in the cytoplasm, nucleus and midbody. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
