| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mineralocorticoid receptor;MR;Nuclear receptor subfamily 3 group C member 2;NR3C2;MCR, MLR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NR3C2, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mineralocorticoid receptor (gene NR3C2) in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 107.1 kDa, and the supplier reports an observed band at about 110 kDa.
The mineralocorticoid receptor (MR, NR3C2) is a nuclear receptor activated by aldosterone and also by cortisol. In kidney epithelia it drives genes that increase sodium and water retention and potassium excretion, raising blood pressure. MR is studied in blood pressure control, heart and kidney disease, and it is the target of spironolactone.
| Target | Mineralocorticoid receptor (gene NR3C2; UniProt P08235, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 107.1 kDa |
| Observed MW | 110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human LNCAP cells, human MCF-7 cells, rat NRK cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Immunogen
Synthetic peptide from the C-terminal region of human NR3C2. It differs from the mouse sequence at one residue and from the rat sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mineralocorticoid receptor in the cytoplasm, nucleus and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhongqiao Lin et al. (2022) Effect of eplerenone on cognitive impairment in spontaneously hypertensive rats. American Journal of Translational Research. PMID: 35836906
