| Field | Specification |
|---|---|
| Target | |
| Alternative names | Amyloid-beta A4 precursor protein-binding family A member 3; Adapter protein X11gamma; Neuron-specific X11L2 protein; Neuronal Munc18-1-interacting protein 3; Mint-3; APBA3; MINT3; X11L2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Mint3/APBA3 recombinant protein (Position: Q62-E544). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes amyloid-beta A4 precursor protein-binding family A member 3 (gene APBA3) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 61.5 kDa, and the supplier reports an observed band at about 69 kDa.
APBA3 (also called Mint3 or X11γ) is an adaptor protein of the X11/Mint family that binds the amyloid precursor protein and may influence its processing. It has also been reported to enhance HIF-1 activity in macrophages by inhibiting FIH (HIF1AN).
| Target | Amyloid-beta A4 precursor protein-binding family A member 3 (gene APBA3; UniProt O96018, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 61.5 kDa |
| Observed MW | 69 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEK293 cells, human HeLa cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Mint3/APBA3 fragment (Gln62–Glu544), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places amyloid-beta A4 precursor protein-binding family A member 3 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in all tissues examined with lower levels in brain and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
