| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Amyloid-beta A4 precursor protein-binding family A member 3; Adapter protein X11gamma; Neuron-specific X11L2 protein; Neuronal Munc18-1-interacting protein 3; Mint-3; APBA3; MINT3; X11L2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Mint3/APBA3 recombinant protein (Position: Q62-E544). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Mint3/APBA3 Antibody Picoband® is an antibody for APBA3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: APBA3 (amyloid beta precursor protein binding family A member 3); UniProt: O96018
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 69 kDa, calculated 108320 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Mint3/APBA3 Antibody Picoband® catalog # A07396-2.
Biological background
Biological context: May modulate processing of the amyloid-beta precursor protein (APP) and hence formation of APP-beta. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN.
Expression and localization notes: cellular localization: Perinuclear region., tissue context: Expressed in all tissues examined with lower levels in brain and testis..
Common research applications
- Western blotting (WB): Compare APBA3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of APBA3 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify APBA3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Amyloid beta A4 precursor protein-binding family A member 3 is a protein that in humans is encoded by the APBA3 gene. It is mapped to 19p13.3. The protein encoded by this gene is a member of the X11 protein family. It is an adapter protein that interacts with the Alzheimer's disease amyloid precursor protein. This gene product is believed to be involved in signal transduction processes. This gene is a candidate gene for Alzheimer's disease.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Perinuclear region.
- Tissue details: Expressed in all tissues examined with lower levels in brain and testis.
- Research category: Interleukins
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
