| Field | Specification |
|---|---|
| Target | |
| Alternative names | MICOS complex subunit MIC60; Cell proliferation-inducing gene 4/52 protein; Mitochondrial inner membrane protein; Mitofilin; p87/89; IMMT; HMP; MIC60; MINOS2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanMitofilin/IMMTrecombinantprotein(Position:D83-K714).HumanIMMT?shares?88.6%?amino?acid?(aa)?sequence?identity?withmouse?IMMT. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects MICOS complex subunit MIC60 (gene IMMT) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 83.7 kDa, and the supplier reports an observed band at about 80–90 kDa.
Mitofilin (MIC60, encoded by IMMT) is the core subunit of the MICOS complex in the mitochondrial inner membrane. It is required for forming crista junctions and contact sites with the outer membrane, so it is essential for normal cristae structure. Mitofilin is widely used as a mitochondrial marker in studies of mitochondrial architecture and dysfunction.
| Target | MICOS complex subunit MIC60 (gene IMMT; UniProt Q16891, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 83.7 kDa |
| Observed MW | 80–90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Colo320 cells, human HepG2 cells, rat NRK cells, rat PC-12 cells, rat C6 cells, mouse HEPA1/6 cells, mouse MFC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human esophageal squamous carcinoma tissue, human rectum adenocarcinoma tissue, human lung cancer tissue, human testicular germ cells tumors tissue, mouse heart tissue, rat heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
E. coli-derivedhumanMitofilin/IMMTrecombinantprotein(Position:D83-K714).HumanIMMT?shares?88.6%?amino?acid?(aa)?sequence?identity?withmouse?IMMT.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places MICOS complex subunit MIC60 in the mitochondrion inner membrane and mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xinna Wang et al. (2025) Xingnao jingshen prescription reduces mitochondrial cristae breakage by protecting GPX1 from oxidation against cerebral ischemia-reperfusion injury. Phytomedicine. 10.1016/j.phymed.2025.157369
