| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitofusin-2;3.6.5.-;Transmembrane GTPase MFN2;MFN2;CPRP1, KIAA0214; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Mitofusin 2 recombinant protein (Position: V601-R757). Human Mitofusin 2 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat Mitofusin 2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mitofusin-2 (gene MFN2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 86.4 kDa, and the supplier reports an observed band at about 86 kDa.
Mitofusin-2 (MFN2) is an outer mitochondrial membrane GTPase that mediates mitochondrial fusion together with MFN1. It also helps tether mitochondria to the endoplasmic reticulum and contributes to mitophagy and mitochondrial transport in axons. Mutations in MFN2 cause Charcot-Marie-Tooth disease type 2A, and it is studied in neurodegeneration and metabolism.
| Target | Mitofusin-2 (gene MFN2; UniProt O95140, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 86.4 kDa |
| Observed MW | 86 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): HeLa cells, A549 cells, rat brain tissue, rat heart tissue, rat kidney tissue, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse small intestine tissue, mouse NIH3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human Mitofusin 2 fragment (Val601–Arg757), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96% and 95%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitofusin-2 in the mitochondrion outer membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hongjing Zhao et al. (2019) Destruction of redox and mitochondrial dynamics co-contributes to programmed cell death in chicken kidney under arsenite or/and copper (II) exposure. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2019.04.062
Hanyue Zheng et al. (2024) Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study. Nanomaterials. 10.3390/nano14080689
Shuo Zhang et al. (2020) Neuroprotective mechanisms of ε-viniferin in a rotenone-induced cell model of Parkinson's disease: significance of SIRT3-mediated FOXO3 deacetylation. Neural Regeneration Research. 10.4103/1673-5374.282264
