| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mitofusin-2;3.6.5.-;Transmembrane GTPase MFN2;MFN2;CPRP1, KIAA0214; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Mitofusin 2 recombinant protein (Position: V601-R757). Human Mitofusin 2 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat Mitofusin 2, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of MFN2 (Mitofusin-2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Mitofusin 2/MFN2 Antibody Picoband® catalog # PB9265. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Mitofusin 2 recombinant protein (Position: V601-R757). Human Mitofusin 2 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat Mitofusin 2, respectively. (reported region: V601-R757).
- Molecular weight context: reported MW: 86 kDa; calculated MW: 86402 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Mitofusin-2; Mitofusin-2. Mitofusin-2 is a protein that in humans is encoded by the MFN2 gene. It is mapped to chromosome 1 and encodes a 757-amino acid protein that contains an ATP/GTP-binding site motif. This gene is expressed in many tissues and cell lines such as brain and KG-1 with the highest expression in heart and skeletal muscle. It has been found that MFN2 triggers mitochondrial energization, at least in part, by regulating OXPHOS expression through signals that are independent of its role as a mitochondrial fusion protein. And it contributes to the maintenance and operation of the mitochondrial network. Axonal CMT type 2A and autosomal dominant HMSN VI are caused by MFN2 and mutations in MFN2, which emphasizes its important role of mitochondrial function for both optic atrophies and peripheral neuropathies. Functional note: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. . Reported localization: Mitochondrion outer membrane ; Multi- pass membrane protein . Colocalizes with BAX during apoptosis. Expression/tissue context: Ubiquitous; expressed at low level. Highly expressed in heart and kidney. .
Research relevance and current trends
- Apoptosis: Researchers commonly examine how MFN2 (Mitofusin-2) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how MFN2 (Mitofusin-2) relates to this theme using model systems and orthogonal readouts.
- Cell Death: Researchers commonly examine how MFN2 (Mitofusin-2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MFN2 (Mitofusin-2) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
