| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myocardin-related transcription factor A; MRTF-A; MKL/myocardin-like protein 1; Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; MRTFA; KIAA1438; MAL; MKL1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Mkl1/MRTFA recombinant protein (Position: L192-L931). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes myocardin-related transcription factor A (gene MRTFA) in human samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 98.9 kDa, and the supplier reports an observed band at about 145 kDa.
MRTF-A (MRTFA, also called MKL1 or MAL) is a transcriptional coactivator of serum response factor (SRF). It binds monomeric G-actin, which keeps it inactive, so Rho-driven actin polymerization releases MRTF-A to enter the nucleus and switch on cytoskeletal genes, linking actin dynamics to gene expression in cell migration and fibrosis.
| Target | Myocardin-related transcription factor A (gene MRTFA; UniProt Q969V6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 98.9 kDa |
| Observed MW | 145 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): SiHa cells.
Immunogen
Recombinant human Mkl1/MRTFA fragment (Leu192–Leu931), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places myocardin-related transcription factor A in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
