Anti-MLX Antibody Picoband®

Overview

Anti-MLX Antibody Picoband® is an antibody for MLX detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey,Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: MLX (aquaporin 5); UniProt: Q9UH92
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 50 kDa, calculated 64406 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-MLX Antibody Picoband® catalog # A03087-2.

Biological background

Biological context: Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).

Expression and localization notes: cellular localization: Apical cell membrane., tissue context: Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood..

Common research applications

• Western blotting (WB): Compare MLX levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of MLX in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify MLX-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Max-like protein X is a protein that in humans is encoded by the MLX gene. The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Apical cell membrane.
• Tissue details: Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.
• Research category: Cancer,Channels,Metabolism,Plasma Membrane,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.