| Field | Specification |
|---|---|
| Target | |
| Alternative names | Monocyte to macrophage differentiation factor; Progestin and adipoQ receptor family member 11; Progestin and adipoQ receptor family member XI; MMD; PAQR11 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MMD recombinant protein (Position: R20-A182). Human MMD shares 100% amino acid (aa) sequence identity with both mouse and rat MMD. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes monocyte to macrophage differentiation factor (gene MMD) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
Monocyte to macrophage differentiation factor (MMD) is a membrane protein of the PAQR family whose expression rises as monocytes mature into macrophages. It is associated with lysosomal membranes, and its precise function has not been fully characterized.
| Target | Monocyte to macrophage differentiation factor (gene MMD; UniProt Q15546, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human MMD fragment (Arg20–Ala182), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 100%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places monocyte to macrophage differentiation factor in the late endosome membrane and lysosome membrane. Tissue expression noted by UniProt (human): Exhibits relatively ubiquitous expression with preferential expression in mature (in vitro differentiated) macrophages.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xuemei Li et al. (2024) Identification and verification of the optimal feature genes of ferroptosis in thyroid-associated orbitopathy. Frontiers in Immunology. 10.3389/fimmu.2024.1422497
