| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interstitial collagenase;3.4.24.7;Fibroblast collagenase;Matrix metalloproteinase-1;MMP-1;22 kDa interstitial collagenase;27 kDa interstitial collagenase;MMP1;CLG; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human MMP1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MMP1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 21M52; isotype IgG; reactivity: Human. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-MMP1 Rabbit Monoclonal Antibody catalog # M00733-2. Tested in WB, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: MMP1 (Interstitial collagenase).
- Antibody format: Monoclonal; clone 21M52; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MMP1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. . Reported cellular localization context: Secreted, extracellular space, extracellular matrix . Tissue expression notes (as provided): Ubiquitous. Expressed at high levels in testis.
Research relevance and current trends
- Research context keywords from the source record include: Angiogenesis,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Metalloprotease,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction,Tumor Biomarkers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate MMP1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MMP1 expression by Western blot in cell or tissue lysates, Enrich MMP1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 54 kDa; calculated MW: 54007 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 54 kDa
- Cellular localization (provided): Secreted, extracellular space, extracellular matrix .
- Tissue details (provided): Ubiquitous. Expressed at high levels in testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
