| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interstitial collagenase;3.4.24.7;Fibroblast collagenase;Matrix metalloproteinase-1;MMP-1;22 kDa interstitial collagenase;27 kDa interstitial collagenase;MMP1;CLG; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MMP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21M52 is a rabbit monoclonal antibody that recognizes interstitial collagenase (gene MMP1) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 54.0 kDa, and the supplier reports an observed band at about 54 kDa.
Interstitial collagenase (MMP-1) is a secreted zinc-dependent matrix metalloproteinase that cuts the triple helix of fibrillar collagens types I, II and III at a single site. This first cut starts collagen breakdown, so MMP-1 is studied in wound healing, arthritis, skin aging and tumor invasion.
| Target | Interstitial collagenase (gene MMP1; UniProt P03956, human) |
|---|---|
| Clone | 21M52 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.0 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MMP1.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Bao et al. (2010) HER2-mediated upregulation of MMP-1 is involved in gastric cancer cell invasion. Archives of Biochemistry and Biophysics. 10.1016/j.abb.2010.05.009
Wendan Yuan et al. (2014) Effects of hydroxysafflor yellow A on proliferation and collagen synthesis of rat vascular adventitial fibroblasts induced by angiotensin II. International Journal of Clinical and Experimental Pathology. PMID: 25337218
