| Field | Specification |
|---|---|
| Target | |
| Alternative names | Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MMP12 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CHE-13 is a rabbit monoclonal antibody that recognizes macrophage metalloelastase (gene MMP12) in human samples and is validated for Western blot. The predicted molecular weight is 54.0 kDa; the supplier reports observed bands at 54, 45, 22 kDa.
Macrophage metalloelastase (MMP-12) is a matrix metalloproteinase produced mainly by macrophages that breaks down elastin and other matrix proteins. It is widely studied in lung tissue injury and remodeling, especially in models of emphysema and chronic inflammation.
| Target | Macrophage metalloelastase (gene MMP12; UniProt P39900, human) |
|---|---|
| Clone | CHE-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.0 kDa |
| Observed MW | 54, 45, 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human MMP12.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Found in alveolar macrophages but not in peripheral blood monocytes.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
