| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human MMP12 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MMP12 Rabbit Monoclonal Antibody is an antibody targeting MMP12. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: CHE-13; isotype: Rabbit IgG; reactivity: Human; observed MW: 43 kDa; calculated MW: 54002 MW.
Boster Bio Anti-MMP12 Rabbit Monoclonal Antibody catalog # M01520. Tested in WB application. This antibody reacts with Human.
Key elements and design rationale
- Target: MMP12 — Macrophage metalloelastase
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: CHE-13; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 43 kDa; Calculated: 54002 MW
Biological background
Protein function (datasheet): May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Cellular localization (datasheet): Secreted, extracellular space, extracellular matrix .
Tissue details (datasheet): Found in alveolar macrophages but not in peripheral blood monocytes.
Research relevance and current trends
- Commonly studied in contexts related to Angiogenesis,Atherosclerosis,Cancer,Cardiovascular,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
