| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagenase 3; Matrix metalloproteinase-13; MMP-13; UMRCASE; Mmp13 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat MMP13 recombinant protein (Position: Y99-H335). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes collagenase 3 (gene Mmp13) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 53.4 kDa, and the supplier reports an observed band at about 54 kDa.
Collagenase 3 (MMP-13) is a matrix metalloproteinase that degrades fibrillar collagens, with the highest activity toward type II collagen of cartilage, and it also cleaves aggrecan and other matrix proteins. It is needed for normal bone development and is widely studied in cartilage breakdown in osteoarthritis.
| Target | Collagenase 3 (gene Mmp13; UniProt P23097, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 53.4 kDa |
| Observed MW | 54 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human PC-3 cells, human U2OS cells, human HEK293 cells, rat testicular tissue, mouse testicular tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, mouse small intestine tissue, rat lung tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant rat MMP13 fragment (Tyr99–His335), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes collagenase 3 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Y. Chen et al. (2022) NIR-driven polydopamine-based nanoenzymes as ROS scavengers to suppress osteoarthritis progression. Materials Today Nano. 10.1016/j.mtnano.2022.100240
Qicui Zhu et al. (2025) Bioinspired exosome-SiO2 nanohybrid therapeutic for rheumatoid arthritis treatment. Theranostics. 10.7150/thno.108296
Liang Shao et al. (2025) Engineering natural arginine finger to deliver phosphate-containing drugs for treating osteoarthritis. Chemical Engineering Journal. 10.1016/j.cej.2025.164353
Haimin Chen et al. (2019) Cartilage-targeting and dual MMP-13/pH responsive theranostic nanoprobes for osteoarthritis imaging and precision therapy. Biomaterials. 10.1016/j.biomaterials.2019.119520
Zhimin Li et al. (2023) High drug loading hydrophobic cross-linked dextran microspheres as novel drug delivery systems for the treatment of osteoarthritis. Asian Journal of Pharmaceutical Sciences. 10.1016/j.ajps.2023.100830
Meisong Zhu et al. (2023) ADRM1/RPN13 attenuates cartilage extracellular matrix degradation via enhancing UCH37-mediated ALK5 deubiquitination. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2023.125670
Ko-Ta Chen et al. (2026) Gluteal adipose-derived stem cell exosomes promote macrophage polarization and cartilage regeneration in osteoarthritis via PTPRC modulation. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2026.119130
