| Field | Specification |
|---|---|
| Target | |
| Alternative names | Matrix metalloproteinase-14;MMP-14;3.4.24.80;MMP-X1;Membrane-type matrix metalloproteinase 1;MT-MMP 1;MTMMP1;Membrane-type-1 matrix metalloproteinase;MT1-MMP;MT1MMP;MMP14; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes matrix metalloproteinase-14 (gene MMP14) in human samples and is validated for Western blot. The predicted molecular weight is 65.9 kDa, and the supplier reports an observed band at about 55 kDa.
MT1-MMP (MMP14) is a membrane-anchored matrix metalloproteinase that degrades collagen and other matrix proteins directly at the cell surface. It also activates pro-MMP-2 and cleaves many other proteins, so it is a key enzyme in skeletal development, angiogenesis and cell invasion.
| Target | Matrix metalloproteinase-14 (gene MMP14; UniProt P50281, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 65.9 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human placenta tissue, HeLa cells, U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human MMP14. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human. UniProt places matrix metalloproteinase-14 in the cell membrane, melanosome and cytoplasm. Tissue expression noted by UniProt (human): Expressed in stromal cells of colon, breast, and head and neck. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yanjie Zhang et al. (2019) Induction of cystathionine gamma-lyase expression and metallothionein-1 S-sulfhydration alleviate cadmium-induced cell death in myoblast cells. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2019.04.063
Ying-Bin Hu et al. (2007) Modified synthetic siRNA targeting tissue inhibitor of metalloproteinase-2 inhibits hepatic fibrogenesis in rats. Journal of Gene Medicine. 10.1002/jgm.1009
Maojun Liu et al. (2018) Hydrogen sulfide ameliorates rat myocardial fibrosis induced by thyroxine through PI3K/AKT signaling pathway. Endocrine Journal. 10.1507/endocrj.EJ17-0445
Shi Chen-hui et al. (2015) Effect of lentivirus-mediated uPA silencing on the proliferation and apoptosis of chondrocytes and the expression of MMPs. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-015-1398-1