| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 72 kDa type IV collagenase;3.4.24.24;72 kDa gelatinase;Gelatinase A;Matrix metalloproteinase-2;MMP-2;TBE-1;PEX;MMP2;CLG4A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human MMP2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MMP2 Rabbit Monoclonal Antibody is an antibody targeting MMP2. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: BBA-13; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 95 kDa; calculated MW: 73882 MW.
Boster Bio Anti-MMP2 Rabbit Monoclonal Antibody catalog # M00286-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: MMP2 — 72 kDa type IV collagenase
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: BBA-13; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 95 kDa; Calculated: 73882 MW
Biological background
Protein function (datasheet): Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.
Cellular localization (datasheet): Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
Tissue details (datasheet): Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. .
Research relevance and current trends
- Commonly studied in contexts related to Tumor Biomarkers,Response To Hypoxia,Proteolytic Enzymes.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
