| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neutrophil collagenase;3.4.24.34;Matrix metalloproteinase-8;MMP-8;PMNL collagenase;PMNL-CL;MMP8;CLG1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MMP8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EFA-13 is a rabbit monoclonal antibody that recognizes neutrophil collagenase (gene MMP8) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 53.4 kDa, and the supplier reports an observed band at about 70 kDa.
Neutrophil collagenase (MMP-8) is a matrix metalloproteinase stored in neutrophil granules and released during inflammation. It cleaves fibrillar collagens types I, II and III and is studied in inflammatory tissue destruction, including periodontal disease.
| Target | Neutrophil collagenase (gene MMP8; UniProt P22894, human) |
|---|---|
| Clone | EFA-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.4 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human placenta.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human spleen tissue, mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human MMP8.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neutrophil collagenase in the cytoplasmic granule. Tissue expression noted by UniProt (human): Neutrophils. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Yaling et al. (2021) H2S improves doxorubicin-induced myocardial fibrosis by inhibiting oxidative stress and apoptosis via Keap1-Nrf2. Technology and Health Care. 10.3233/THC-218020
Xiaorui Zhou et al. (2014) Matrix metalloproteinase-8 inhibitors mitigate sepsis-induced myocardial injury in rats. Chinese Medical Journal. 10.3760/cma.j.issn.0366-6999.20132143
Li Jian et al. (2004) Effects of 17 β-estradiol on the expression of interstitial collagenases-8 and −13 (MMP-8 and MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in ovariectomized rat osteoblastic cells. Journal of Molecular Histology. 10.1007/s10735-004-6206-3
