| Field | Specification |
|---|---|
| Target | |
| Alternative names | WD repeat domain-containing protein 83;Mitogen-activated protein kinase organizer 1;MAPK organizer 1;WDR83;MORG1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Morg1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets WD repeat domain-containing protein 83 (gene WDR83) in rat, mouse and human samples and is validated for Western blot and IHC. The predicted molecular weight is 34.3 kDa, and the supplier reports an observed band at about 12 kDa.
WDR83, also known as MORG1, is a WD-repeat scaffold protein that helps organize multiprotein signaling complexes. It supports assembly of the ERK MAP kinase scaffold, binds the prolyl hydroxylase PHD3 to modulate HIF-1α levels under hypoxia, and has been linked to cell polarity and to restraining mTORC1 activity, so it appears in studies of signaling, hypoxia and autophagy.
| Target | WD repeat domain-containing protein 83 (gene WDR83; UniProt Q9BRX9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 34.3 kDa |
| Observed MW | 12 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat, Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
Samples with a confirmed band (WB): rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of human morg1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt places WD repeat domain-containing protein 83 in the cytoplasm, lysosome and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sophie Wulf et al. (2023) Targeted Disruption of the MORG1 Gene in Mice Causes Embryonic Resorption in Early Phase of Development. Biomolecules. 10.3390/biom13071037
Yan Chen et al. (2019) Oxymatrine reverses epithelial-mesenchymal transition in breast cancer cells by depressing αⅤβ3 integrin/FAK/PI3K/Akt signaling activation. OncoTargets and Therapy. 10.2147/OTT.S209056
