| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53 |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target |
Overview
Anti-Mouse TP53/p53 Antibody (PAb242) targets TP53/p53 (Cellular tumor antigen p53). Frequently used across ELISA, IF, IHC, IP, RIA, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Oncology & Angiogenesis research.
Also known as: Cellular tumor antigen p53, Phosphoprotein p53, Tumor suppressor p53, Antigen NY-CO-13, TP53, P53.
Target biology
TP53/p53 (Cellular tumor antigen p53) is annotated as cellular tumor antigen p53 and is commonly profiled for expression and localization in relevant model systems. It is frequently investigated in contexts related to Oncology & Angiogenesis. Common alternative names and symbols used in the literature include: Phosphoprotein p53, Tumor suppressor p53, Antigen NY-CO-13, TP53, P53. Based on the annotated name/class, the target is most often associated with context-dependent localization, though this can vary by model system.
How this antibody helps
As a monoclonal reagent, this antibody is designed for clone-to-clone consistency and epitope-focused recognition. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.
Recommended experimental notes
- Titrate the antibody to optimize signal-to-noise for your specific sample type and detection system.
- Use appropriate positive and negative controls (e.g., target-high vs target-low samples, KO/KD where available).
- For Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.
- For IHC, optimize fixation and antigen retrieval (buffer/pH/heat) and include an isotype/secondary-only control to assess background.
- For IF/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.
- For immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.
- For ELISA, optimize capture/detection pairing and run a standard curve in matrix-matched diluent when possible.
Additional notes
For research use only.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
