| Field | Specification |
|---|---|
| Target | |
| Alternative names | Multidrug resistance-associated protein 1;ATP-binding cassette sub-family C member 1;Leukotriene C (4) transporter;LTC4 transporter;ABCC1;MRP, MRP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MRP1, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ATP-binding cassette sub-family C member 1 (gene ABCC1) in human samples and is validated for Western blot. The predicted molecular weight is 171.6 kDa, and the supplier reports an observed band at about 220 kDa.
Multidrug resistance-associated protein 1 (ABCC1, MRP1) is an ATP-powered pump that exports organic anions conjugated to glutathione, glucuronate or sulfate out of cells. It also carries glutathione and the inflammatory mediator leukotriene C4, and its overexpression contributes to drug resistance in cancer.
| Target | ATP-binding cassette sub-family C member 1 (gene ABCC1; UniProt P33527, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 171.6 kDa |
| Observed MW | 220 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HeLa cells, A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human MRP1. It differs from the mouse sequence at five residues and from the rat sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ATP-binding cassette sub-family C member 1 in the cell membrane and basolateral cell membrane. Tissue expression noted by UniProt (human): Lung, testis and peripheral blood mononuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Samikkannu Thangavel et al. (2018) Oxidative Stress in HIV Infection and Alcohol Use: Role of Redox Signals in Modulation of Lipid Rafts and ATP-Binding Cassette Transporters. Antioxidants & Redox Signaling. 10.1089/ars.2016.6830
