| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Multidrug resistance-associated protein 4;ATP-binding cassette sub-family C member 4;MRP/cMOAT-related ABC transporter;Multi-specific organic anion transporter B;MOAT-B;ABCC4;MRP4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human MRP4 recombinant protein (Position: M1-K77). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ABCC4 (Multidrug resistance-associated protein 4) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-MRP4/ABCC4 Antibody Picoband® catalog # A01596. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human MRP4 recombinant protein (Position: M1-K77). (reported region: M1-K77).
- Molecular weight context: reported MW: 200-250 kDa; calculated MW: 149527 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Multidrug resistance-associated protein 4; Multidrug resistance-associated protein 4. ABCC4 (Atp-binding cassette, subfamily c, member 4), also known as MRP4 or MOATB, is a protein that in humans is encoded by the ABCC4 gene. It belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. This gene is mapped to chromosome 13q32. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus. The antiproliferative effect of ABCC4 inhibition was related to cAMP-dependent PKA activation and CREB phosphorylation. Pharmacologic inhibition of ABCC4 activity or knockdown of ABCC4 via RNA interference resulted in reduced migration of DCs from human skin explants and of in vitro-generated Langerhans cells. It has been found that ABCC4 contributes to migration of DCs toward draining lymph nodes and therefore has a role in the initiation of an immune response. Functional note: May be an organic anion pump relevant to cellular detoxification. Reported localization: Membrane; Multi-pass membrane protein. Expression/tissue context: Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
Research relevance and current trends
- ATPases: Researchers commonly examine how ABCC4 (Multidrug resistance-associated protein 4) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how ABCC4 (Multidrug resistance-associated protein 4) relates to this theme using model systems and orthogonal readouts.
- Channels: Researchers commonly examine how ABCC4 (Multidrug resistance-associated protein 4) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ABCC4 (Multidrug resistance-associated protein 4) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ABCC4 (Multidrug resistance-associated protein 4) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
