| Field | Specification |
|---|---|
| Target | |
| Alternative names | Small ribosomal subunit protein uS15m; 28S ribosomal protein S15, mitochondrial; MRP-S15; S15mt; MRPS15; RPMS15 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MRPS15 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27M80 is a rabbit monoclonal antibody that recognizes small ribosomal subunit protein uS15m (gene MRPS15) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 29.8 kDa, and the supplier reports an observed band at about 22 kDa.
MRPS15 is a nuclear-encoded protein of the small subunit of the mitochondrial ribosome, which translates the mtDNA-encoded components of the oxidative phosphorylation system.
| Target | Small ribosomal subunit protein uS15m (gene MRPS15; UniProt P82914, human) |
|---|---|
| Clone | 27M80 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.8 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MRPS15.
Reactivity Notes
The supplier lists reactivity with human. UniProt places small ribosomal subunit protein uS15m in the mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
