| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ribosome-recycling factor, mitochondrial; RRF; mtRRF; Ribosome-releasing factor, mitochondrial; MRRF |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MRRF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27M02 is a rabbit monoclonal antibody that recognizes ribosome-recycling factor, mitochondrial (gene MRRF) in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 29.3 kDa, and the supplier reports an observed band at about 24 kDa.
Mitochondrial ribosome recycling factor (MRRF) acts at the end of mitochondrial protein synthesis. Working with the elongation factor GFM2, it splits the mitoribosome into its subunits and releases it from the mRNA so that it can start a new round of translation.
| Target | Ribosome-recycling factor, mitochondrial (gene MRRF; UniProt Q96E11, human) |
|---|---|
| Clone | 27M02 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.3 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MRRF.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places ribosome-recycling factor, mitochondrial in the mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
