| Field | Specification |
|---|---|
| Target | |
| Alternative names | Disintegrin and metalloproteinase domain-containing protein 8; ADAM 8; Cell surface antigen MS2; CD antigen CD156a; ADAM8; MS2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MS2/ADAM8 recombinant protein (Position: R41-L290). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets disintegrin and metalloproteinase domain-containing protein 8 (gene ADAM8) in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 88.8 kDa, and the supplier reports an observed band at about 80 kDa.
ADAM8 is a metalloprotease-disintegrin expressed mainly in immune cells such as neutrophils and macrophages. It cleaves adhesion molecules and cytokine receptors and is thought to support leukocyte migration into tissues, so it is studied in inflammation and cancer.
| Target | Disintegrin and metalloproteinase domain-containing protein 8 (gene ADAM8; UniProt P78325, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 88.8 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Raji cells, human Caco-2 cells, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human MS2/ADAM8 fragment (Arg41–Leu290), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places disintegrin and metalloproteinase domain-containing protein 8 in the membrane. Tissue expression noted by UniProt (human): Expressed on neutrophils and monocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
