| Field | Specification |
|---|---|
| Target | |
| Alternative names | Paired amphipathic helix protein Sin3a; Histone deacetylase complex subunit Sin3a; Transcriptional corepressor Sin3a; SIN3A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human mSin3A/SIN3A recombinant protein (Position: Q8-E918). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects paired amphipathic helix protein Sin3a in human and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 145.2 kDa, and the supplier reports an observed band at about 150 kDa.
SIN3A is a large scaffold protein at the core of the SIN3–HDAC corepressor complex, which also contains HDAC1 and HDAC2. Recruited by repressors such as MAD/MXI1–MAX and REST, it silences target genes through histone deacetylation, and it takes part in MYC antagonism, circadian gene repression and neural development.
| Target | Paired amphipathic helix protein Sin3a (gene SIN3A; UniProt Q96ST3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 145.2 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human 293T cells, human K562 cells, human MCF-7 cells, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human larynx squamous cells carcinoma tissue, human lung adenocarcinoma tissue, human ovarian serous cancer tissue, human prostate adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with confirmed staining (ICC/IF): human ovarian cancer tissue.
Samples with a confirmed signal (flow cytometry): 293T cells, SiHa cells.
Immunogen
Recombinant human mSin3A/SIN3A fragment (Gln8–Glu918), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places paired amphipathic helix protein Sin3a in the nucleus. Tissue expression noted by UniProt (human): Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yaqi Qiu et al. (2025) The transcriptional regulator Sin3a activates CD44 and promotes collective luminal breast cancer cell migration. Journal of Biological Chemistry. 10.1016/j.jbc.2025.110264
