| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hepatocyte growth factor-like protein; Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MST1; D3F15S2; DNF15S2; HGFL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MST1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFBA-13 is a rabbit monoclonal antibody that recognizes hepatocyte growth factor-like protein (gene MST1) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 80.3 kDa, and the supplier reports an observed band at about 85 kDa.
Macrophage-stimulating protein (MSP, encoded by MST1, also called hepatocyte growth factor-like protein) is a plasminogen-related growth factor made mainly by the liver and secreted as an inactive precursor. Once cleaved, it binds and activates the receptor tyrosine kinase RON (MST1R) to regulate macrophage activity, cell migration and epithelial responses. It is distinct from the Hippo pathway kinase also called MST1 (STK4).
| Target | Hepatocyte growth factor-like protein (gene MST1; UniProt P26927, human) |
|---|---|
| Clone | AFBA-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 80.3 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human MST1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes hepatocyte growth factor-like protein as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
