| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide II; MT-CO2; COII; COX2; COXII; MTCO2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MT-CO2 recombinant protein (Position: M1-S205). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cytochrome c oxidase subunit 2 (gene MT-CO2) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 25.6 kDa, and the supplier reports an observed band at about 21 kDa.
Cytochrome c oxidase subunit 2 (MT-CO2, COX2) is a mitochondrial DNA-encoded core subunit of complex IV of the respiratory chain. It contains the copper A center that accepts electrons from cytochrome c and passes them to subunit 1, where oxygen is reduced to water.
| Target | Cytochrome c oxidase subunit 2 (gene MT-CO2; UniProt P00403, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 25.6 kDa |
| Observed MW | 21 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human MCF-7 cells, human HeLa cells, human Jurkat cells, human A431 cells, human Hacat cells, rat liver tissue, rat brain tissue, rat small intestine tissue, rat RH35 cells, mouse liver tissue, mouse brain tissue, mouse small intestine tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colorectal cancer tissue, human glioma tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human MT-CO2 fragment (Met1–Ser205), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytochrome c oxidase subunit 2 in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiangjun Li et al. (2020) Effect of environmental tobacco smoke on COX-2 and SHP-2 expression in a periodontitis rat model. Oral Diseases. 10.1111/odi.13538
Yanmei Jin et al. (2007) Interactive actions of prostaglandin and epidermal growth factor to enhance proliferation of granulosa cells from chicken prehierarchical follicles. Prostaglandins & Other Lipid Mediators. 10.1016/j.prostaglandins.2007.02.002
Zhu Jing et al. (2018) Effect of maternal folic acid supplementation on prostatitis risk in the rat offspring. International Urology and Nephrology. 10.1007/s11255-018-1969-8
Yue TENG et al. (2018) Anti-inflammatory effect of tranexamic acid against trauma-hemorrhagic shock-induced acute lung injury in rats. Experimental Animals. 10.1538/expanim.17-0143
