| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADH-ubiquinone oxidoreductase chain 1; NADH dehydrogenase subunit 1; MT-ND1; MTND1; NADH1; ND1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MT-ND1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23M01 is a rabbit monoclonal antibody that recognizes NADH-ubiquinone oxidoreductase chain 1 (gene MT-ND1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 35.7 kDa, and the supplier reports an observed band at about 38 kDa.
MT-ND1 is a mitochondrial DNA-encoded core subunit of complex I (NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain. Complex I passes electrons from NADH to ubiquinone while pumping protons, and MT-ND1 is needed for its assembly and activity. Mutations in MT-ND1 are associated with Leber hereditary optic neuropathy.
| Target | NADH-ubiquinone oxidoreductase chain 1 (gene MT-ND1; UniProt P03886, human) |
|---|---|
| Clone | 23M01 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.7 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human fetal muscle.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MT-ND1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NADH-ubiquinone oxidoreductase chain 1 in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
