| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-1-tetrahydrofolate synthase, cytoplasmic; C1-THF synthase; Epididymis secretory sperm binding protein; MTHFD1; MTHFC; MTHFD |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MTHFD1 recombinant protein (Position: K21-Q855). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets C-1-tetrahydrofolate synthase, cytoplasmic (gene MTHFD1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 101.6 kDa, and the supplier reports an observed band at about 102 kDa.
MTHFD1 is a cytoplasmic trifunctional enzyme of folate-mediated one-carbon metabolism, combining methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase activities. It interconverts one-carbon forms of tetrahydrofolate that feed purine, thymidylate and methionine synthesis, and is studied in nutrition, development and cancer metabolism.
| Target | C-1-tetrahydrofolate synthase, cytoplasmic (gene MTHFD1; UniProt P11586, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 101.6 kDa |
| Observed MW | 102 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL/ml (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL/ml (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL/ml (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/mL/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL/ml (Human) |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, rat brain tissue, mouse brain tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human adenocarcinoma of the lung tissue, human chronic tonsillitis tissue, human diffuse large B cells lymphoma tissue, human liver cancer tissue, human thyroid cancer tissue, rat liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with confirmed staining (ICC/IF): human tonsil tissue.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human MTHFD1 fragment (Lys21–Gln855), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places C-1-tetrahydrofolate synthase, cytoplasmic in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
