| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microsomal triglyceride transfer protein large subunit; MTTP; MTP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MTTP recombinant protein (Position: N29-R702). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes microsomal triglyceride transfer protein large subunit (gene MTTP) in human, monkey and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 99.4 kDa, and the supplier reports an observed band at about 99 kDa.
Microsomal triglyceride transfer protein (MTTP) is an endoplasmic reticulum lipid transfer protein that works with protein disulfide isomerase. It loads triglyceride, cholesteryl ester and phospholipid onto apolipoprotein B, a step required to assemble and secrete VLDL in the liver and chylomicrons in the intestine. Loss-of-function mutations cause abetalipoproteinemia.
| Target | Microsomal triglyceride transfer protein large subunit (gene MTTP; UniProt P55157, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 99.4 kDa |
| Observed MW | 99 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/m (Human) |
Samples with a confirmed band (WB): human HepG2 cells, monkey liver tissue, rat liver tissue, rat small intestine tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, rat liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HepG2 cells.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human MTTP fragment (Asn29–Arg702), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey and rat. UniProt places microsomal triglyceride transfer protein large subunit in the endoplasmic reticulum and Golgi apparatus. Tissue expression noted by UniProt (human): Liver and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kenan Peng et al. (2023) Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr−/− mice. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2023.166850
Qian Du et al. (2023) Metabolic characteristics of voriconazole – Induced liver injury in rats. Chemico-Biological Interactions. 10.1016/j.cbi.2023.110693
