| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucin-1; MUC-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227; Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-beta; MUC1-CT; MUC1; PUM |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human MUC1 recombinant protein (Position: S1062-V1139). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mucin-1 (gene MUC1) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 122.1 kDa; the supplier reports observed bands at 120, 200 kDa.
Mucin-1 (MUC1) is a heavily glycosylated transmembrane mucin on the apical surface of most epithelia, forming part of the protective barrier. Its cytoplasmic tail engages signaling pathways including ERK, SRC and NF-κB and can influence p53-regulated transcription. MUC1 is often overexpressed and aberrantly glycosylated in carcinomas, so it is widely studied in cancer biology.
| Target | Mucin-1 (gene MUC1; UniProt P15941, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 122.1 kDa |
| Observed MW | 120, 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human ovarican cancer tissue, mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human MUC1 fragment (Ser1062–Val1139), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mucin-1 in the apical cell membrane. Tissue expression noted by UniProt (human): Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guiling Yang et al. (2020) Imidacloprid disturbed the gut barrier function and interfered with bile acids metabolism in mice. Environmental Pollution. 10.1016/j.envpol.2020.115290
Zhang Jianjun et al. (2013) Establishment of a new representative model of human ovarian cancer in mice. Journal of Ovarian Research. 10.1186/1757-2215-6-9
Huaiping Shi et al. (2014) Establishment and characterization of a dairy goat mammary epithelial cell line with human telomerase (hT-MECs). Animal Science Journal. 10.1111/asj.12206
