| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucin-1;MUC-1;Breast carcinoma-associated antigen DF3;Cancer antigen 15-3;CA 15-3;Carcinoma-associated mucin;Episialin;H23AG;Krebs von den Lungen-6;KL-6;PEMT;Peanut-reactive urinary mucin;PUM;Polymorphic epithelial mucin;PEM;Tumor-associated epithelial membrane antigen;EMA;Tumor-associated mucin;CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;MUC1;PUM; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MUC1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FHA-13 is a rabbit monoclonal antibody that recognizes mucin-1 (gene MUC1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 122.1 kDa, and the supplier reports an observed band at about 17–30 kDa.
Mucin-1 (MUC1) is a heavily glycosylated transmembrane mucin on the apical surface of most epithelia, forming part of the protective barrier. Its cytoplasmic tail engages signaling pathways including ERK, SRC and NF-κB and can influence p53-regulated transcription. MUC1 is often overexpressed and aberrantly glycosylated in carcinomas, so it is widely studied in cancer biology.
| Target | Mucin-1 (gene MUC1; UniProt P15941, human) |
|---|---|
| Clone | FHA-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 122.1 kDa |
| Observed MW | 17–30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human MCF-7 cells, human HeLa cells, rat lung tissue, rat stomach tissue, mouse lung tissue, mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cnacer tissue, human ovarian cnacer tissue, mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human MUC1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mucin-1 in the apical cell membrane. Tissue expression noted by UniProt (human): Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Ling et al. (2026) Prostaglandin E2-driven dedifferentiation of Schwann cells leads to perineural invasion in pancreatic ductal adenocarcinoma. Signal Transduction and Targeted Therapy. 10.1038/s41392-026-02648-x
Mengchao Ding et al. (2022) “Cocktail” anti-tumor strategy through enhanced antigen exposure with photothermal/chemodynamic therapy. Chemical Engineering Journal. 10.1016/j.cej.2022.141091
Lin Xiaolan et al. (2024) Efficacy of MUC1-targeted CAR-NK cells against human tongue squamous cell carcinoma. Frontiers in Immunology. 10.3389/fimmu.2024.1337557
Bihui Wang et al. (2025) The role of SIGLEC9 in immunosuppression and prognosis in cervical cancer. Clinics. 10.1016/j.clinsp.2025.100849
