| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucin-16 ;MUC-16;Ovarian cancer-related tumor marker CA125 ;CA-125 ;Ovarian carcinoma antigen CA125 ;MUC16 ;CA125 ; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MUC16 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GOG-13 is a rabbit monoclonal antibody that recognizes mucin-16 (gene MUC16) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 1, 519.2 kDa, and the supplier reports an observed band at about 1519 kDa.
Mucin-16 (MUC16) is a very large transmembrane mucin that forms part of the protective glycocalyx on ocular, respiratory and reproductive tract epithelia. Its shed extracellular portion is the CA125 antigen, a long-established serum marker used in ovarian cancer research.
| Target | Mucin-16 (gene MUC16; UniProt Q8WXI7, human) |
|---|---|
| Clone | GOG-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 1, 519.2 kDa |
| Observed MW | 1519 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human ovary cancer.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MUC16.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes mucin-16 as secreted and also located in the cell membrane. Tissue expression noted by UniProt (human): Expressed in corneal and conjunctival epithelia (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Du Li-Cheng et al. (2014) VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma. Reproductive Biology and Endocrinology. 10.1186/1477-7827-12-14
