| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucin-2;MUC-2;Intestinal mucin-2;MUC2;SMUC; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MUC2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAEO-13 is a rabbit monoclonal antibody that recognizes mucin-2 (gene MUC2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 540.3 kDa, and the supplier reports an observed band at about 110 kDa.
Mucin-2 (MUC2) is the main gel-forming mucin secreted by intestinal goblet cells. Its large polymeric networks build the colonic mucus layer that separates bacteria from the epithelium while allowing nutrient uptake, and it has also been shown to bind copper. MUC2 is a standard goblet cell marker in studies of gut barrier function, colitis and the microbiome.
| Target | Mucin-2 (gene MUC2; UniProt Q02817, human) |
|---|---|
| Clone | AAEO-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 540.3 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human Caco-2 cells, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon tissue, human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human MUC2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes mucin-2 as a secreted protein. Tissue expression noted by UniProt (human): Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Manchun Huang et al. (2025) Arabinoxylan from barley bran alleviates ulcerative colitis in mice through enhancement of mucosal barrier function and modulation of gut microbiota. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.144941
Chen Qian et al. (2023) Evaluation of two laboratory model methods for diarrheal irritable bowel syndrome. Molecular Medicine. 10.1186/s10020-022-00599-x
Zhang Xuan et al. (2021) Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora. Frontiers in Microbiology. 10.3389/fmicb.2020.622354
Wenyan Zhou et al. (2025) TRPV4 agonist GSK1016790A increases mucus production and alleviates diabetic intestinal injury by activating the Ca2+/CAMKII/GSK3β pathway. European Journal of Pharmacology. 10.1016/j.ejphar.2025.178481
Yapeng Yang et al. (2026) Fecal microbiota transplantation from healthy piglets ameliorates intestinal inflammation in mice by modulating recipient metabolism. BMC Microbiology. 10.1186/s12866-025-04590-4
Kasahara Naoya et al. (2024) Dietary polyamines promote intestinal adaptation in an experimental model of short bowel syndrome. Scientific Reports. 10.1038/s41598-024-55258-4
Lu Jiang et al. (2026) Hederagenin Alleviates Ulcerative Colitis via Suppressing the Activation of EGFR/p38 MAPK Signaling. Journal of Gastroenterology and Hepatology. 10.1111/jgh.70403
De-Kun Wu et al. (2024) A mechanistic study of Anwei decoction intervention in a rat model of gastric intestinal metaplasia through the endoplasmic reticulum stress–autophagy pathway. Tissue & Cell. 10.1016/j.tice.2024.102317
Yu Zhou et al. (2022) Liangxue Tongyu Prescription Alleviates Brain Damage in Acute Intracerebral Hemorrhage Rats by Regulating Intestinal Mucosal Barrier Function. Evidence-based Complementary and Alternative Medicine. 10.1155/2022/2197763
