| Field | Specification |
|---|---|
| Target | |
| Alternative names | Muscarinic acetylcholine receptor M3; CHRM3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18C00 is a rabbit monoclonal antibody that recognizes muscarinic acetylcholine receptor M3 (gene CHRM3) in human samples and is validated for Western blot. The predicted molecular weight is 66.1 kDa, and the supplier reports an observed band at about 66 kDa.
The muscarinic acetylcholine receptor M3 (CHRM3) is a G protein-coupled receptor that signals through Gq proteins to raise intracellular Ca2+. It drives smooth muscle contraction in the airways, bladder and gut and stimulates glandular secretion, making it an important target in pharmacology.
| Target | Muscarinic acetylcholine receptor M3 (gene CHRM3; UniProt P20309, human) |
|---|---|
| Clone | 18C00 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 66.1 kDa |
| Observed MW | 66 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human fetal brain.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human muscarinic Acetylcholine Receptor M3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places muscarinic acetylcholine receptor M3 in the cell membrane, postsynaptic cell membrane, basolateral cell membrane and endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fenglin Sun et al. (2019) Acacetin-induced cell apoptosis in head and neck squamous cell carcinoma cells: Evidence for the role of muscarinic M3 receptor. Phytotherapy Research. 10.1002/ptr.6343
