| Field | Specification |
|---|---|
| Target | |
| Alternative names | Methylmalonyl-CoA mutase, mitochondrial; MCM; Methylmalonyl-CoA isomerase; MMUT; MUT |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MUTA |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27M55 is a rabbit monoclonal antibody that recognizes methylmalonyl-CoA mutase, mitochondrial (gene MMUT) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 83.1 kDa, and the supplier reports an observed band at about 83 kDa.
Methylmalonyl-CoA mutase (MMUT) is a mitochondrial enzyme that uses adenosylcobalamin, an active form of vitamin B12, to convert methylmalonyl-CoA into succinyl-CoA. This reaction feeds products of branched-chain amino acid and odd-chain fatty acid breakdown into the TCA cycle, and MMUT deficiency causes methylmalonic acidemia.
| Target | Methylmalonyl-CoA mutase, mitochondrial (gene MMUT; UniProt P22033, human) |
|---|---|
| Clone | 27M55 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.1 kDa |
| Observed MW | 83 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human K562 cells, human 293T cells, human PC-3 cells, rat PC-12 cells, rat kidney tissue, mouse NIH/3T3 cells, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MUTA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places methylmalonyl-CoA mutase, mitochondrial in the mitochondrion matrix, mitochondrion and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
