| Field | Specification |
|---|---|
| Target | |
| Alternative names | Diphosphomevalonate decarboxylase; Mevalonate (diphospho)decarboxylase; MDDase; Mevalonate pyrophosphate decarboxylase; MVD; MPD |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MVD |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27M00 is a rabbit monoclonal antibody that recognizes diphosphomevalonate decarboxylase (gene MVD) in human samples and is validated for Western blot. The predicted molecular weight is 43.4 kDa, and the supplier reports an observed band at about 43 kDa.
Diphosphomevalonate decarboxylase (MVD) catalyzes the ATP-dependent decarboxylation of mevalonate 5-diphosphate to isopentenyl diphosphate. This is the last step of the mevalonate pathway, which supplies building blocks for cholesterol, other sterols and isoprenoids, and is studied in lipid metabolism and prenylation.
| Target | Diphosphomevalonate decarboxylase (gene MVD; UniProt P53602, human) |
|---|---|
| Clone | 27M00 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.4 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MVD.
Reactivity Notes
The supplier lists reactivity with human. UniProt places diphosphomevalonate decarboxylase in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in heart, skeletal muscle, lung, liver, brain, pancreas, kidney and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
