| Field | Specification |
|---|---|
| Target | |
| Alternative names | Major vault protein; MVP; Lung resistance-related protein; MVP; LRP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MVP recombinant protein (Position: A2-H259). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 8B12 is a mouse monoclonal antibody that recognizes major vault protein (gene MVP) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 99.3 kDa, and the supplier reports an observed band at about 100–110 kDa.
Major vault protein (MVP) is the main structural component of vaults, very large barrel-shaped ribonucleoprotein particles in the cytoplasm. Many MVP copies assemble into the vault shell, which also contains PARP4, TEP1 and small vault RNAs. Vaults have been linked to transport and signaling, including dampening of interferon-γ/STAT1 signaling, and to drug resistance.
| Target | Major vault protein (gene MVP; UniProt Q14764, human) |
|---|---|
| Clone | 8B12 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 99.3 kDa |
| Observed MW | 100–110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human A549 tissue, human U2OS cells, human HepG2 cells, human THP-1 cells, human HeLa cells, human SW620 whole cellrat RH35 whole cellmouse RAW2467 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, human colon cancer tissue, rat intestine tissue, human mammary cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): A549 cells, A431 cells.
Immunogen
Recombinant human MVP fragment (Ala2–His259), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places major vault protein in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Present in most normal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wan Mingyue et al. (2023) Study on immunogenicity of Lrp subunit vaccine against Vibrio alginolyticus in pearl gentian grouper(♀Epinephelus fuscoguttatus ×♂Epinephelus lanceolatus). Frontiers in Marine Science. 10.3389/fmars.2023.1098816
Yuhong Ye et al. (2026) TIE2 Interacts with Major Vault Protein and Modulates Angiogenic Function in Human Retinal Microvascular Endothelial Cells Through PI3K/AKT Signaling. Current Eye Research. 10.1080/02713683.2026.2653072
