Description
Anti-Myc Affinity Gel is prepared by conjugating high quality murine IgG1 monoclonal antibody with Sepharose 4B gel. This product has a high Myc label fusion protein loading capacity (at least 0.9 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Myc labels.
Features
- Specific—highly specific monoclonal antibody
- High yield and high purity
- Versatile—It can be used for protein purification or COIP
- Convenient—reagents and peptides to elute are available separately
Applications
- Immunoprecipitation (IP)
- Purification of fusion proteins with Myc labels.
Specifications
| Clone | 9E10 |
| Isotype | Mouse IgG1 |
| Purity | Protein A |
| Antibody Concentration | 8.5 g antibody/L Gel |
| Protein binding capacity | ≥0.9 mg protein/mL Gel |
| Storage buffer | 10 mM Na3PO4, 150mM NaCl, 50% glycerin, pH7.4, contains 0.02% (w/v) sodium azide |
Components
| Components No. | Name | 20587ES03 | 20587ES08 | 20587ES25 | 20587ES60 |
| 20587 | Anti-Myc Affinity Gel | 1 mL | 5 mL | 25 mL | 100 mL |
Storage
The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Do not freeze in solution without glycerin!
The antibody is clone 9E10, isotype Mouse IgG1, purified by Protein A. Antibody concentration: 8.5 g antibody/L gel. Clone 9E10 is the gold-standard anti-Myc monoclonal antibody recognizing the human c-Myc epitope EQKLISEEDL. It does not cross-react with the endogenous mouse or human c-Myc protein at typical loading concentrations.
Compatible with N-terminal, C-terminal, or internal Myc (EQKLISEEDL) tags. Tandem Myc-tagged proteins (2×Myc) bind with higher efficiency. The antibody does not reliably capture other epitope tags. Note: this product captures tagged proteins via the Myc epitope, not endogenous c-Myc oncoprotein.
The gel provides a binding capacity of ≥0.9 mg Myc-tagged fusion protein per mL of gel — lower than the HA (≥1.5 mg/mL) and His (≥1.1 mg/mL) versions. This should be considered when designing pull-down experiments with high-abundance Myc-tagged targets; scale bead volume accordingly.
Preferred: Myc peptide elution (EQKLISEEDL at 1 mg/mL) for native, functional protein recovery. Alternative: low-pH glycine (pH 2.5–3.0) for non-peptide elution; neutralize immediately. SDS sample buffer for gel analysis only.
Unopened: store at −25°C to −15°C for 1 year. Do not freeze without glycerol-containing buffer. After opening, store at 2–8°C. Storage buffer: 10 mM Na₃PO₄, 150 mM NaCl, 50% glycerol, pH 7.4, 0.02% sodium azide.
The following customization and add-on services may be available for this product type through the supplier. For inquiries and pricing, contact support@biohippo.com.
Customization Options
- Bulk Quantities: Larger volumes and custom pack sizes beyond the standard catalog may be available for high-throughput core facilities and industrial customers. Contact us to inquire about bulk pricing.
- Custom Antibody Coupling (Magnetic Beads): Custom magnetic beads conjugated to your specific antibody or protein ligand may be available through Yeasen's OEM and custom services program. These services leverage the same bead platform used for catalog products.
- OEM Manufacturing: Custom bead formulations for diagnostic device development, including magnetic beads for nucleic acid isolation, IP-grade beads, and affinity capture beads, may be available at manufacturing scale.
To inquire about custom options or bulk pricing, contact support@biohippo.com.
