| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myeloid differentiation primary response protein MyD88; Myd88; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse MyD88 recombinant protein (Position: S12-D263). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects myeloid differentiation primary response protein MyD88 in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 33.8 kDa, and the supplier reports an observed band at about 34 kDa.
MyD88 is the adaptor that links Toll-like receptors and IL-1 family receptors to downstream kinases in innate immunity. Through IRAK1, IRAK2 and TRAF6 it drives NF-κB activation and the release of inflammatory cytokines, so it is a common readout in studies of infection, sterile inflammation and TLR-targeted drugs.
| Target | Myeloid differentiation primary response protein MyD88 (gene Myd88; UniProt P22366, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 33.8 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): rat liver tissue, rat lung tissue, rat PC-12 cells, rat RH35 cells, mouse liver tissue, mouse lung tissue, mouse RAW264.7 cells, mouse ANA-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant mouse MyD88 fragment (Ser12–Asp263), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places myeloid differentiation primary response protein MyD88 in the cytoplasm and nucleus. Tissue expression noted by UniProt (mouse): Detected in bone marrow. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chuanbo Sun et al. (2024) Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118067
Hao Wu et al. (2024) Chaihu Guizhi Ganjiang Decoction attenuates nonalcoholic steatohepatitis by enhancing intestinal barrier integrity and ameliorating PPARα mediated lipotoxicity. Journal of Ethnopharmacology. 10.1016/j.jep.2024.117841
Ying Li et al. (2023) UHPLC-MS-Based Metabolomics Reveal the Potential Mechanism of Armillaria mellea Acid Polysaccharide in and Its Effects on Cyclophosphamide-Induced Immunosuppressed Mice. Molecules. 10.3390/molecules28247944
Dan Song et al. (2024) The mechanism of baicalin in improving pulmonary inflammatory response and injury and regulating intestinal flora in Mycoplasma pneumoniae pneumonia mice. Cellular Signalling. 10.1016/j.cellsig.2024.111530
Zhiyong Long et al. (2021) Exploring the Mechanism of Resveratrol in Reducing the Soft Tissue Damage of Osteoarthritis Based on Network Pharmacology and Experimental Pharmacology. Evidence-based Complementary and Alternative Medicine. 10.1155/2021/9931957
