| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myogenic factor 5; Myf-5; Class C basic helix-loop-helix protein 2; bHLHc2; MYF5 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MYF5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24M01 is a rabbit monoclonal antibody that recognizes myogenic factor 5 (gene MYF5) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 28.3 kDa, and the supplier reports an observed band at about 37 kDa.
Myf5 (myogenic factor 5) is a bHLH transcription factor of the MyoD family. It is one of the earliest determinants of skeletal muscle lineage, marking muscle progenitors and quiescent satellite cells and activating muscle-specific genes together with MyoD and myogenin. It is widely used as a marker in myogenesis and muscle regeneration studies.
| Target | Myogenic factor 5 (gene MYF5; UniProt P13349, human) |
|---|---|
| Clone | 24M01 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 28.3 kDa |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human skeletal muscle.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MYF5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places myogenic factor 5 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
