| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myosin-11;Myosin heavy chain 11;Myosin heavy chain, smooth muscle isoform;SMMHC;MYH11;KIAA0866; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Myosin, smooth muscle heavy chain 1 and 2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFAC-13 is a rabbit monoclonal antibody that recognizes myosin-11 (gene MYH11) in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 227.3 kDa, and the supplier reports an observed band at about 250 kDa.
Smooth muscle myosin heavy chain (MYH11) is the motor protein of smooth muscle thick filaments, converting ATP hydrolysis into contraction. It is a specific marker of mature smooth muscle cells, and MYH11 mutations are linked to familial thoracic aortic aneurysm, while a CBFB-MYH11 fusion occurs in a subtype of acute myeloid leukemia.
| Target | Myosin-11 (gene MYH11; UniProt P35749, human) |
|---|---|
| Clone | AFAC-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 227.3 kDa |
| Observed MW | 250 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human bladder.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human myosin, smooth muscle heavy chain 1 and 2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places myosin-11 in the melanosome. Tissue expression noted by UniProt (human): Smooth muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Qin et al. (2019) Compound heterozygous variants in MYH11 underlie autosomal recessive megacystis-microcolon-intestinal hypoperistalsis syndrome in a Chinese family. Journal of Human Genetics. 10.1038/s10038-019-0651-z
