| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myosin-7B; Antigen MLAA-21; Myosin cardiac muscle beta chain; Myosin heavy chain 7B, cardiac muscle beta isoform; Slow A MYH14; MYH7B; KIAA1512 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MYH7B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25M38 is a rabbit monoclonal antibody that recognizes myosin-7B (gene MYH7B) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 225.8 kDa, and the supplier reports an observed band at about 221 kDa.
Myosin-7B (MYH7B) is a sarcomeric myosin heavy chain gene expressed at low levels in heart and some skeletal muscles. Its intron hosts the microRNA miR-499, and the protein is studied in muscle biology, although its role in contraction is less defined than other cardiac myosins.
| Target | Myosin-7B (gene MYH7B; UniProt A7E2Y1, human) |
|---|---|
| Clone | 25M38 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 225.8 kDa |
| Observed MW | 221 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal muscle.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MYH7B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places myosin-7B in the membrane. Tissue expression noted by UniProt (human): Expressed in heart, skeletal muscle, testis, and all specific brain regions examined. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
