| Field | Specification |
|---|---|
| Target | |
| Alternative names | Unconventional myosin-VIIa; MYO7A; USH1B |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Myosin VIIa Myosins |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFCH-13 is a rabbit monoclonal antibody that recognizes unconventional myosin-VIIa (gene MYO7A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 254.4 kDa, and the supplier reports an observed band at about 254 kDa.
Myosin VIIa (MYO7A) is an unconventional myosin expressed in inner ear hair cells and retinal pigment epithelium. It helps organize hair cell stereocilia and moves melanosomes and phagosomes in the retina. Mutations cause Usher syndrome type 1B and non-syndromic deafness, making it a key protein in hearing and vision research.
| Target | Unconventional myosin-VIIa (gene MYO7A; UniProt Q13402, human) |
|---|---|
| Clone | AFCH-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 254.4 kDa |
| Observed MW | 254 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): Y79 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human myosin VIIa Myosins.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places unconventional myosin-VIIa in the cytoplasm and synapse. Tissue expression noted by UniProt (human): Expressed in the pigment epithelium and the photoreceptor cells of the retina. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Ya’nan et al. (2026) Eleutheroside E alleviates cisplatin-induced ototoxicity by down-regulating MAPK/NF-κB/NLRP3 signaling pathway and inhibiting cochlear cell pyroptosis. Communications Biology. 10.1038/s42003-025-09490-x
